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*10.19 A 10-kb genomic DNA EcoRI fragment from a newly discovered insect is ligated into the EcoRI site of the pBluescript II plasmid vector (described in Figure8.4, p. 176) and transformed into E. coli. Plasmid DNA and genomic DNA from the insect are prepared and each DNA sample is digested completely with the restrictionenzyme EcoRI. The two digests are loaded into separate wells of an agarose gel, and electrophoresis is used to separate the products by size.a. What will be seen in the lanes of the gel after it is stained to visualize the size-separated DNA molecules?b. What will be seen if the gel is transferred to a membraneto make a Southern blot, and the blot is probed with the 10-kb EcoRI fragment? (Assume the fragment does not contain any repetitive DNA sequence.)
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