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A difference between bacterial and eukaryotic transcription RNA is synthesized 5' to 3' in bacteria, but 3' to 5' in eukaryotes. transfer RNAs (tRNAs) are involved in eukaryotic transcription only. in bacteria, the start signal is an AUG, while in eukaryotes it is a promoter. in bacteria, introns have to be removed from the primary RNA transcript. in eukaryotes, there are different RNA polymerases (I, II, and III).

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