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After transforming competent E. coli cells with a few microliter of the ligation mix containing plasmid and PCR insert, you plated the cells on LB agar containing ampicillin. The next day you are excited to see as many as 450 colonies on your plate! You now need to grow small cultures from some of these colonies so that you can isolate the recombinant plasmid from the selected cultures and sequence it. You thought the colony PCR you learned in class was really cool, but this is not a standard procedure in the lab where you work. Which one of the arguments below is correct for convincing your supervisor you should perform colony PCR?

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