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Date - Data Protection Act and GDPR - Failure to protect data, disclose without consent, re-identify anonymous data, editing information.
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- The epithelial cells that line the intestine are organized in structures called crypts and vili. The Crypts are recessed while the vili extend into the intestinal lumen. The intestinal epithelia have short lifespans (on the order of a week) and they are replaced by an intestinal stem cell located at the very bottom of the crypt. These stem cells are marked by a protein called LGR5.You wish to use the CreER approach discussed in class to confirm that LGR5-expressing cells really do give rise to all epithelial cells. How could you do this in a mouse? Describe how long you would activate CreER and how long you would wait to assay. Justify your experimental setup. Remember that CreER is activated by a drug and is only active when the drug is around.
- Mr. Smith has a prescription for diazepam 5mg and would like to know if you have it in stock. How may you help the patient?a. Ask him to come back later because you have to put in the prescription before you can tell if you have it in stockb. Ask the pharmacist to check the inventory because it a C-IV controlled medicationc. Check the inventory on the shelf and report its availabilityd. Tell him that you are not authorized to give out such information.
- Type of outcome: faster, less expensive to measure (i.e. bone density change during a 3-yr study vs lifetime risk of vertebral fracture)