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• De manière spécifique => étude du génome notamment sur l'étude des gènes ARN ribosomiques 16S (très conservés) grâce au PCR, séquençage, banque de données...En deux groupes => celles Gram+ (=membrane plasmique + paroi très épaisse) et celle Gram- (=membrane plasmique + paroi fine + membrane plasmique)Comment ? Coloration violette, décoloration alcool (gram - décolorées), recoloration à la fushine => Gram+ reste violette et Gram- roseEx: Gram- => ESCHERICHIA coli (Bacille)Gram+ => STAPHILOCOCCIS aureus (Cocci)
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