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For relaxation to occur:calcium leaves the sarcoplasmic reticulum, troponin binds calcium and blocks the binding sites on actin. calcium enters the myofibril, it detaches from myosin, and the binding sites on myosin become inactive. sarcoplasm calcium levels fall, calcium is removed from troponin, and tropomyosin blocks binding sites on actin. extracellular calcium levels fall, calcium is pumped into the myofibril, and tropomyosin slides away from actin.
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