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How is positive supercoiling in replication resolved?
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- With two containers of a liquid connected by a tube also full of liquid, the difference in height between the liquid levels will drive movement of liquid from one container to the other-when both ends are open to the atmosphere, the net downward pressure is P_net = P_2 - P_1 = pgdelta(H)-when one end is open to the atmosphere, P_flask + pg(deltaH) = P_atm, so P_flask < P_atm ~to make P_flask = P_atm, make deltaH = 0