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Pectinase is a protein that catalyzes the breakdown of pectic polysaccharides in plant cell walls. A researcher designs an experiment to investigate the effect of salinity on the ability of pectinase to lower the activation energy of the reaction involved. The design of the experiment is presented in Table 1. For each test tube, the researcher will measure the amount of product formed over 20 minutes.Test Tube Sodium Chloride Concentration (molar) Temperature (degrees C) Substrate Added Pectinase Added1 0 23 Yes No2 0 23 Yes Yes3 0.5 23 Yes No4 0.5 23 Yes Yes5 1.0 23 Yes No6 1.0 23 Yes Yes7 1.5 23 Yes No8 1.5 23 Yes YesWhich of the following statements best helps justify the inclusion of test tube 5 in the experiment?
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