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Photosynthetic organisms use elaborate machinery to incorporate carbon (as CO2) into glyceraldehyde-3- phosphate, which is used to synthesize glucose for later metabolism. However, one carbon is lost following glycolysis when the three-carbon pyruvate is converted to acetyl-CoA. The bacterial enzyme pyruvate-formate lyase (also known as formate C-acetyltransferase) catalyzes the reaction [see image]. How does this reaction help the cell avoid losing carbon?

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