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QUANTIFICATION DES TRANSCRITS PAR PCR QAUNTITATIVE=qPCRObjectif: Mesurer la _________ d'ADN dans la matrice initiale Principe: -___________ de la quantité d'ADN amplifié à chaque cycle pendant la phase exponentielle -Mesure de la quantité d'ADN grâce à un marqueur ____________(mesure en temps réel par la machine de l'intensité du signal de fluorescence en fonction des cycles de PCR)Différence avec la PCR: PCR en temps réel= au fur et à mesure de la réaction
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