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Since the laboratory analysis of homocysteine is complicated by the fact that it exists bound to other thiol amino acids via disulfide bonds, it is first necessary to subject these disulfide bonds to reduction prior to analysis. It is necessary to reduce these disulfide bonds in order to get a true value of the quantity of homocysteine in a plasma sample. Why is this so?Coice A The true quantity of homocysteine would be artificially high if the reduction step were not carried out prior to analysis.(Choice B, It is necessary because disulfide bound homocysteine would otherwise precipitate out of the serum before analysis could be conducted.(Choice C, The true quantity of homocysteine would be artificially low if the reduction step were not carried out prior to analysis.(Choice D, It is necessary in order to prevent homocysteine from degrading in the sample.
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