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Specimen: culture blood or CFS (7-10days), urine (1st week)Transport: immediate material transportation: Sterile container, For CFS thayer martin, urine uroline, blood hemolineCultivation: selective enrichment broth, semisolid culture mediumDirect:Darkfield microscopy (silver stain), PCR, IFIndirect:Serodiagnostics: Microscopic agglutination test (MAT), indirect ELISA, Indirect IF, indirect hemoagglutination test
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