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Specimen: pus, tissue biopsy- sputum (induced, bronchoalveolar lavage)Transportaton: sterile container, transport media for anaerobesCultvaton: 37 degrees, 5 - 20 days, under anaerobic / microaerophilic conditons (anerobic chambers, anaerobic jars) - chocolate / other enriched medium for example brucella or blood agar can be usedIdentificaton:Microscopy: Gram staining, with microscopy Gr+ filamentous rods can be detectedBiochemical properties: API, VITEKMolecular testing: PCR, MALDI-TOF, sequencingAntbiotc susceptibility: VITEK, E test, disk diffusion test (suscep,ble to wide range of beta - lactams)
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