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Specimen: Sputum, bronchoalveolar lavage urine, blood, feces, surgical materialTransportation: Sterile container, amies, vacutainer blood tubesCultivation:- Löwenstein jensen medium- Bactec MGITDirect identifactionGeneXpert testMicroscopy: Liquid media: fast acid bacteria (carbolfuchin stain used or ziel niehlsen/acid fast stain->red rods, blue background - sputum)Indirect:Biochemical properties: VITEK, APIMolecular diagnostics:Latent TBInterferon-γ test (IGRA tests)Serologiacl identification: : ELISAAntibiotic susceptibility: VITEK, Disc disk diffusion, E-test, PCRaddition: X-ray, Tuberculin-test
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