Back to Questions
Want to know:
The Cas9 is a protein enzyme that cuts DNA at targets specified by a short guide RNA bound to it. The sgRNA includes a 20-nucleotide sequence that is complementary to the DNA strand that it will cut. If the target DNA site has to be perfectly complementary to all 20 nucleotides, approximately how many sites will be cut in the human genome for a particular sgRNA? The human genome is 3 billion (3 x 10E9) nucleotides per haploid set of chromosomes.In other, very pertinent terms for human gene therapy, if you design an sgRNA to cut at a particular site in a human gene, how many off-target sites do you think the sgRNA-Cas9 complex will cut in the rest of the genome? (Set up and solve the problem with your group.)
Get a detailed, AI-powered explanation for this question and thousands more on StudyFetch.
Get the Answer for FreeHow StudyFetch Helps You Master This Topic
AI-Powered Answers
Get instant, detailed explanations powered by AI that understands your course material.
Deep Understanding
Go beyond surface-level answers with step-by-step breakdowns and examples.
Personalized Learning
Spark.E adapts to your learning style and helps you connect ideas.
Practice & Test
Turn any question into flashcards, quizzes, and practice tests to solidify your knowledge.
Explore More Questions
- Based on this passage, how did Thoreau feel about his confinement?
- Lecture 2- so the idea of universals (basic truths) is SUPER important for the moral life! These are called
- Which of the following is not a type of ion transporter that has been observed? a. The sodium/calcium exchanger b. The sodium/potassium/chloride co-transporter c. The sodium/neurotransmitter co-transporter d. The sodium/proton exchanger e. All of the above transporters have been observed.