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There is only one start codon, AUG. This means that all newly-made polypeptides have a methionine at their carboxyl end. the first tRNA will have the anticodon loop 3'-AUG-5'. all newly-made polypeptides have a methionine at their amino end. the stop codons each have their own special tRNAs. the 5' end of an mRNA must start with an A.
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