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Today we will select colonies that grew from individual E. coli cells in the tubes to which we added 2.5 uL ligation reaction. After we let the cells sit on ice for 10 min., we let the cells recover in SOC medium at 37C, and spread the cells on solid medium (beads!) for overnight growth. Given what you have learned about the plasmid we are using in MCB4034L, how did we make sure that only E. coli cells that have taken up a plasmid molecule survived?

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