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Vectors: Plasmid and Virus (the mechanism for putting recombinant DNA in an organism)Extract required gene from human chromosomeRestriction enzymes cut DNA at restriction sitesLigase enzymes 'glue' sticky-ends of gene and plasmid togetherMakes recombinant DNAPut recombinant DNA in an organism - makes a TRANSGENIC organismGrow TRANSGENIC organism with tissue culture or fermenter.Used to make human insulin for diabetics
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