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What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?A: Extract plasmid DNA from bacterial cells. Cut the plasmid DNA using restriction enzymes. Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments. Use ligase to seal plasmid DNA to nonplasmid DNA. Transform bacteria with a recombinant DNA molecule.B: Extract plasmid DNA from bacterial cells. Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments. Use ligase to seal plasmid DNA to nonplasmid DNA. Transform bacteria with a recombinant DNA molecule. Cut the plasmid DNA using restriction enzymes.C: Transform bacteria with a recombinant DNA molecule. Cut the plasmid DNA using restriction enzymes. Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments. Extract plasmid DNA from bacterial cells. Use ligase to seal plasmid DNA to nonplasmid DNA.D: Cut the plasmid DNA using restriction enzymes. Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments. Use ligase to seal plasmid DNA to nonplasmid DNA. Extract plasmid DNA from bacterial cells. Transform bacteria with a recombinant DNA molecule.
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